Screening of Musa balbisiana Colla. seeds for antidiabetic properties and isolation of apiforol, a potential lead, with antidiabetic activity
Greeshma Gopalan, B. Prabha, Alfred Joe, T. R. Reshmitha, D. R. Sherin, Billu Abhraham M. Sabu,* T. K. Manojkumar,* K. V. Radhakrishnan* and P. Nisha*
Seeds of the edible fruits of Musa balbisiana, Colla. were investigated for its antidiabetic potential. After estimating the proximate composition, the seeds were extracted with various solvents and evaluated for its antidiabetic potential in terms of the inhibition of digestive enzymes, antiglycation activity and in vitro glucose uptake. The acetone extract demonstrated the highest inhibition of a-amylase and ?-glucosidase enzyme with an IC50 value of 36.67 ± 0.367 µg/mL and 100.61 ± 0.707 µg/mL respectively. The extract also exhibited significant glycation inhibition property with an IC50 value of 86.48 ± 0.751 µg/mL. Furthermore, a major phytochemical, apiforol was isolated from the acetone extract for the first time, which demonstrated promising a-glucosidase inhibition (IC50 = 48.25 ± 0.255 µM), antiglycation property (IC50 = 114.23 ± 0.567 µM) and enhanced glucose uptake in L6 myoblasts. In the Molecular docking studies, apiforol efficiently bonded to the active sites of a-glucosidase enzyme, 3A4A.
J. Sci. Food Agric., 2018 (Accepted)