Elizabeth Kuruvilla, Danaboyina Ramaiah
The present invention provides the acridinium derivatives represented by Formula 1 as fluorescent marker for the detection and quantitation of single strand DNA under physiological pH conditions and in presence of double strand DNA, proteins and micelles. These derivatives are highly soluble in the aqueous medium, extremely stable in the dark and under irradiation conditions. They exhibit good absorption and fluorescence emission properties with quantitative fluorescence yields. The novelty of this invention is that the acridinium derivatives of Formula 1 can efficiently differentiate single strand DNA from double strand DNA and effective quantitation of single strand DNA can be achieved even in presence of impurities usually present in nucleic acid preparations. Accordingly, these acridinium derivatives are extremely useful as fluorescent marker for the detection and quantitation of single strand DNA, oxidative DNA damage in plasmids and cells and for time resolved monitoring of the hybridization of DNA.