Biosynthesis of 2,5-Furan Dicarboxylic Acid by Aspergillus Flavus APLS-1: Process Optimization and Intermediate Product Analysis
RAJESH (R O), GODAN (T K), Rai (A K), SAHOO (D), PANDEY (A), BINOD (P)
2,5-furan dicarboxylic acid; Aspergillus flavus; Biotransformation; Green bioprocess; Hydroxymethyl furfuraldehyde
- •First report on the production of FDCA by Aspergillus sp.
- •FDCA production of 0.83?g/L (6.6?mM) from 1?g/L (8?mM) HMF.
- •HMF to FDCA conversion achieved with 67% efficiency.
The aim of the present study was to develop an eco-friendly biological process for the production of 2,5-furan dicarboxylic acid (FDCA) from 5-hydroxy methylfurfuraldehyde (HMF) using microorganisms. Microorganisms were isolated from the soil samples and evaluated for its biotransformation efficiency. Among the isolates, Aspergillus flavus APLS-1 was found to be potent for efficient conversion of HMF to FDCA. The bioconversion parameters were optimized by Box-Behnken design. The optimization resulted in 67% conversion efficiency where 1?g/L HMF (8?mM) was transformed to 0.83?g/L (6.6?mM) FDCA in 14?days at pH6.5 with biomass size of 5.7?g/L and biomass age 60?h. This is the first report on Aspergillus sp., capable of detoxifying HMF and produces FDCA.