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Biosynthesis of 2,5-Furan Dicarboxylic Acid by Aspergillus Flavus APLS-1: Process Optimization and Intermediate Product Analysis

Fig. 1. Optimization of FDCA production by using single parameters optimization (a) pH;â?¦


•First report on the production of FDCA by Aspergillus sp.
•FDCA production of 0.83?g/L (6.6?mM) from 1?g/L (8?mM) HMF.
•HMF to FDCA conversion achieved with 67% efficiency.

The aim of the present study was to develop an eco-friendly biological process for the production of 2,5-furan dicarboxylic acid (FDCA) from 5-hydroxy methylfurfuraldehyde (HMF) using microorganisms. Microorganisms were isolated from the soil samples and evaluated for its biotransformation efficiency. Among the isolates, Aspergillus flavus APLS-1 was found to be potent for efficient conversion of HMF to FDCA. The bioconversion parameters were optimized by Box-Behnken design. The optimization resulted in 67% conversion efficiency where 1?g/L HMF (8?mM) was transformed to 0.83?g/L (6.6?mM) FDCA in 14?days at pH6.5 with biomass size of 5.7?g/L and biomass age 60?h. This is the first report on Aspergillus sp., capable of detoxifying HMF and produces FDCA.