Tailoring of Microbes for the Production of High Value Plant-derived Compounds: from Pathway Engineering to Fermentative Production
MADHAVAN (A), ARUN (K B), SINDHU (R), BINOD (P), KIM (S H), PANDEY (A)
plant natural compounds; microbial cell factory; bioprocess; metabolic engineering; fermentation; heterologous proteins
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
1867(11): 140262 https://doi.org/10.1016/j.bbapap.2019.140262
Plant natural products have been an attracting platform for the isolation of various active drugs and other bioactives. However large-scale extraction of these compounds is affected by the difficulty in mass cultivation of these plants and absence of strategies for successful extraction. Even though, synthesis by chemical method is an alternative method; it is less efficient as their chemical structure is highly complex which involve enantio-selectivity. Thus an alternate bio-system for heterologous production of plant natural products using microbes has emerged. Advent of various omics, synthetic and metabolic engineering strategies revolutionised the field of heterologous plant metabolite production. In this context, various engineering methods taken to synthesise plant natural products are described with an additional focus to fermentation strategies.