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Organism and Process for Production of Beta Glucosidase (BGL), A Critical Component in Enzyme Cocktail for Biomass Hydrolysis

CSIR-NIIST had isolated a filamentous fungus from decaying wood in 2005, which was identified as Aspergillus niger and designated isolate no. NII 08121. The fungus produced a BGL which was active at 0.5M glucose and had a glucose inhibition constant (Ki) of 0.2M. The technology developed is a solid-state fermentation process for the production of NII 08121 BGL.

Beta-Glucosidase is an important component in the enzyme (cellulase) cocktail for hydrolysis of biomass to generate sugars for biofuels and other applications, as this is the rate-limiting enzyme. The availability of enzymes for biomass hydrolysis is important for the future energy security indigenous technologies for enzyme production are warranted. CSIR-NIIST has a solid-state fermentation (SSF) technology for the production of BGL using the fungus Aspergillus niger demonstrated successfully at a semi-pilot scale (up-to 10kg level). The technology would help to produce BGL at a cheaper rate than existing processes and would help in development of efficient biomass hydrolyzing enzyme cocktails for biorefinery applications.

beta_glucosidase_for_biofuel_application.pdf   beta_glucosidase_for_biofuel_application.pdf (457.5 KiB, 332 hits)