Study of cavity size and nature of bridging units on recognition of nucleotides by cyclophanes(Org. Biomol. Chem., 2011, 9, 1021-1029.). Cyclophanes containing anthracene units linked together through different bridging and spacer groups have been synthesized and investigated their interactions with various nucleosides and nucleotides. They showed selectivity for 5′-GTP and 5′-ATP as compared to other nucleotides and nucleosides and exhibited significant binding interactions with the fluorescent indicator, 8-hydroxy-1,3,6-pyrene trisulfonate (HPTS), resulting in the formation of non-fluorescent complexes.