Integrated 2G ethanol pilot plant

NIIST’s Pilot plant for bioethanol from lignocellulosicbiomass was commissioned in 2012 and is one of the first 2G ethanol pilot plants in the country. The plant has all operations from biomass milling to ethanol distillation and dehydration. Capacity = 80 kg biomass/batch.

High pressure pretreatment reactor – 1000L (Helical impeller, direct steam injection and jacket circulation)

Neutralization reactor – 750L (Paddle impeller)

Vibra-sifter (Solid liquid Separation) a Plate and Frame Filter

Hydrolysis Reactor – 500L (Paddle impeller, Jacketed)

Fermenter – 200L (150L working volume) and all accessories Distillation unit

The entire plant and individual equipment are available for contract R&D and scale up studies

Solid State Fermentation (SSF)Pilot Plant (Koji Room)

NIIST’s SSF pilot plant has the capacity to operate 100 production scale trays (~50kg moldy bran per batch). This is a fully climate-controlled chamber for operation at pre-decided temperature and humidity. This is a unique facility and one of the very few in the country.

Clean room with vinyl walls

HEPA filtered Air In& Out

Temperature range – 20 C to Ambient +5 C

Humidity – 40 – 85 % RH

BSL2 inoculation facility

800L capacity autoclave

Pilot scale Ceramic Membrane Filtration system for protein concentration – 5kDa filtration

The entire plant and individual equipment are available for contract R&D and scale up studies

Fermentation facility

MPTD has fermenters ranging in volume from 0.5L capacity to 20L (Infors and B Braun/Sartorius), and with all utilities and accessories.

This would allow scale up of laboratory processes and the expertise in chemical engineering would aid process development and optimization. The facilities are available for contract R&D and our expertise is available for consultation.

DNA Sequencer

Applied Biosystems, Genetic Analyzer 3500 Sanger Sequencing

DNA sequencing is routinely done for samples from the division and outside. DNA sequencing is offered as a service and can be requested here

Ultracentrifuge

Beckman Optima XPN 100 (Max Speed – 100000 rpm, 802000 x g)Rotors

100Ti Fixed Angle: 100000 rpm (802000xg) - 8 x 6ml

70Ti Fixed Angle: 70000 rpm (504000xg) – 8 x 39ml

SW41Ti Swing Bucket: 41000 rpm(288000xg)-6 x 13.2 ml